| Title | Gene expression profile of Dclk1+ cells in intestinal tumors. |
| Publication Type | Journal Article |
| Year of Publication | 2018 |
| Authors | Yamaga Y, Fukuda A, Nakanishi Y, Goto N, Matsumoto Y, Yoshioka T, Maruno T, Chiba T, Seno H |
| Journal | Dig Liver Dis |
| Volume | 50 |
| Issue | 12 |
| Pagination | 1353-1361 |
| Date Published | 2018 Dec |
| ISSN | 1878-3562 |
| Keywords | Animals, Doublecortin-Like Kinases, Intestinal Mucosa, Intestinal Neoplasms, Mice, Microarray Analysis, Neoplastic Stem Cells, Phosphorylation, Protein Serine-Threonine Kinases, Proto-Oncogene Proteins c-hck, Receptors, G-Protein-Coupled, src-Family Kinases, Transcriptome |
| Abstract | BACKGROUND: Accumulating evidence has shown the existence of tumor stem cells with therapeutic potential. Previously, we reported that doublecortin like kinase 1 (Dclk1) marks tumor stem cells but not normal stem cells in the intestine of ApcMin/+ mice, and that Dclk1- and Lgr5-double positive tumor cells are the tumor stem cells of intestinal tumors. AIM: To investigate molecules highly expressed in the Dclk1+ normal intestinal and Dclk1+ tumor cells in ApcMin/+ mice. METHODS: We used microarray analyses to examine the gene expression profile of Dclk1+ cells in both mouse normal intestinal epithelium and ApcMin/+ mouse intestinal tumors. We also performed immunofluorescence analyses. RESULTS: Genes related to microtubules and the actin cytoskeleton (e.g., Rac2), and members of the Src family kinases (i.e., Hck, Lyn, Csk, and Ptpn6) were highly expressed in both Dclk1+ normal intestinal and Dclk1+ tumor cells. Phosphorylated Hck and phosphorylated Lyn were expressed in Lgr5+ cells in the intestinal tumors of Lgr5EGFP-IRES-CreERT2/+; ApcMin/+ mice. CONCLUSION: We revealed factors that are highly expressed in Dclk1+ intestinal tumor cells, which may help to develop cancer stem cell-targeted therapy in future. |
| DOI | 10.1016/j.dld.2018.06.011 |
| Alternate Journal | Dig Liver Dis |
| PubMed ID | 30001952 |